3F36
Apoferritin: complex with 2-isopropylphenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.366, 182.366, 182.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.18672 |
| Rwork | 0.185 |
| R-free | 0.20986 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1xz1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.367 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | REFMAC (5.4.0074) |
| Refinement software | REFMAC (5.4.0074) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.540 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.057 | 0.317 |
| Number of reflections | 29075 | |
| <I/σ(I)> | 24.9 | 5.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 23.8 | 11.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Cadmium sulfate, ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
