3F35
Apoferritin: complex with 2,6-diethylphenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.929, 182.929, 182.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.920 - 1.920 |
| R-factor | 0.19254 |
| Rwork | 0.190 |
| R-free | 0.23573 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1xz1 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.751 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | REFMAC (5.4.0074) |
| Refinement software | REFMAC (5.4.0074) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.079 | 0.433 |
| Number of reflections | 20594 | |
| <I/σ(I)> | 18.4 | 5.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 28.32 | 28.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | cadmium sulfate, ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
