3F33
Apoferritin: complex with propofol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.487, 182.487, 182.487 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.110 - 1.700 |
| R-factor | 0.19041 |
| Rwork | 0.189 |
| R-free | 0.21988 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1xz1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.230 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | REFMAC (5.4.0074) |
| Refinement software | REFMAC (5.4.0074) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.250 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.319 |
| Number of reflections | 29110 | |
| <I/σ(I)> | 23.5 | 4.9 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 16.54 | 9.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Cadmium sulfate, ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
