3F2V

Crystal structure of the general stress protein 14 (TDE0354) in complex with FMN from Treponema denticola, Northeast Structural Genomics Consortium Target TdR58.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-10-21
Detector	RIGAKU RAXIS IV
Spacegroup name	C 2 2 21
Unit cell lengths	59.058, 89.622, 69.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.230 - 2.000
R-factor	0.204
Rwork	0.204
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qr2
RMSD bond length	0.012
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP (& COMO)
Refinement software	CNS (1.2 & XtalView)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.042	0.162
Number of reflections	24036
<I/σ(I)>	42.2	8.4
Completeness [%]	99.2	92.2
Redundancy	7.3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbatch under oil	7	277	Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, and 5 mM DTT. Reservoir solution: 50 mM HEPES (pH 7), 20% PEG 3350, and 1% Tryptone., microbatch under oil, temperature 277K