3F2O
Crystal Structure of human splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1) in complex with a 20-residue VASA peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-10 |
| Detector | MAR225 |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.665, 80.654, 86.864 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.240 - 2.050 |
| R-factor | 0.18215 |
| Rwork | 0.180 |
| R-free | 0.23198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jk9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.497 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.240 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.092 | 0.649 |
| Number of reflections | 34014 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG3350, 0.2M NaCl, 0.1M BisTris pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
