3F2M
Urate oxidase complexed with 8-azaxanthine at 150 MPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID27 |
| Synchrotron site | ESRF |
| Beamline | ID27 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2006-12-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.374 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.700, 95.870, 104.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.820 - 1.800 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21800 |
| Starting model (for MR) | 2iba |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.669 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.936 | 19.940 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
| Rmerge | 0.052 | 0.041 | 0.262 |
| Total number of observations | 3097 | 13831 | |
| Number of reflections | 36047 | ||
| <I/σ(I)> | 7.86 | 12.8 | 2.5 |
| Completeness [%] | 96.4 | 92.3 | 96.6 |
| Redundancy | 2.7 | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 3.2MG/ML URATE OXIDASE, EQUIMOLAR CONCENTRATION 8-AZAXANTHINE, 6% PEG 8000, 50mM Tris, 100mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
