3F2M
Urate oxidase complexed with 8-azaxanthine at 150 MPa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID27 |
Synchrotron site | ESRF |
Beamline | ID27 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2006-12-14 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.374 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.700, 95.870, 104.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.820 - 1.800 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.21800 |
Starting model (for MR) | 2iba |
RMSD bond length | 0.016 |
RMSD bond angle | 1.669 |
Data reduction software | XDS |
Data scaling software | SCALA (3.2.21) |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.936 | 19.940 | 1.900 |
High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
Rmerge | 0.052 | 0.041 | 0.262 |
Total number of observations | 3097 | 13831 | |
Number of reflections | 36047 | ||
<I/σ(I)> | 7.86 | 12.8 | 2.5 |
Completeness [%] | 96.4 | 92.3 | 96.6 |
Redundancy | 2.7 | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 3.2MG/ML URATE OXIDASE, EQUIMOLAR CONCENTRATION 8-AZAXANTHINE, 6% PEG 8000, 50mM Tris, 100mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 300K |