3F2E
Crystal structure of Yellowstone SIRV coat protein C-terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Collection date | 2008-08-12 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.542 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 54.410, 54.410, 77.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.642 - 1.668 |
R-factor | 0.1846 |
Rwork | 0.184 |
R-free | 0.20180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CS-Rosetta model |
RMSD bond length | 0.005 |
RMSD bond angle | 0.833 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.588 | 1.700 |
High resolution limit [Å] | 1.668 | 1.668 |
Rmerge | 0.034 | 0.203 |
Number of reflections | 14062 | |
<I/σ(I)> | 33.4 | 8.27 |
Completeness [%] | 99.1 | 94.6 |
Redundancy | 16.8 | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 299 | SIRV CP (residues 46-134) at 19 or 30 mg/ml in 20 mM Na MES pH 6.0 was mixed 1:1 with 25% PEG 20,000, 0.1 M Na Citrate pH 3.6, 4-9% sucrose. Crystals were frozen in of 14% PEG 20,000, 50 mM sodium citrate pH 3.6, 15-25% sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 299K |