3F2D
DNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP, Mn and Zn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-17 |
| Wavelength(s) | 0.999887 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 115.997, 139.479, 184.074 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.150 - 2.510 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f2b |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.701 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.150 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.087 | 0.642 |
| Number of reflections | 50821 | |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 4.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 289 | 20% PEG 2000 MME, 0.1M PHOSPHATE CITRATE, 200MM LITHIUM SULFATE, 1 mM ZnCl2, 2 mM MnCl2, pH 5.30, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2000 MME | ||
| 10 | 1 | 2 | MnCl2 | ||
| 2 | 1 | 1 | PHOSPHATE CITRATE | ||
| 3 | 1 | 1 | LITHIUM SULFATE | ||
| 4 | 1 | 1 | ZnCl2 | ||
| 5 | 1 | 1 | MnCl2 | ||
| 6 | 1 | 2 | PEG 2000 MME | ||
| 7 | 1 | 2 | PHOSPHATE CITRATE | ||
| 8 | 1 | 2 | LITHIUM SULFATE | ||
| 9 | 1 | 2 | ZnCl2 |
