3F2B

DNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP, Mg and Zn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-17
Wavelength(s)	0.999887
Spacegroup name	C 2 2 21
Unit cell lengths	116.174, 140.793, 184.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.520 - 2.390
R-factor	0.231
Rwork	0.228
R-free	0.27300
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.609
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.4.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.520	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.101	0.824
Number of reflections	59793
<I/σ(I)>	10.2
Completeness [%]	99.9	100
Redundancy	5.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3	289	20% PEG 2000 MME, 0.1M PHOSPHATE- CITRATE, 200MM LITHIUM SULFATE, PH 5.3, VAPOR DIFFUSION, pH 5.30, VAPOR DIFFUSION, SITTING DROP, temperature 289K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 2000 MME
2	1	1	PHOSPHATE- CITRATE
3	1	1	LITHIUM SULFATE
4	1	2	PEG 2000 MME
5	1	2	PHOSPHATE- CITRATE
6	1	2	LITHIUM SULFATE