3F1Z
Crystal structure of putative nucleic acid-binding lipoprotein (YP_001337197.1) from Klebsiella pneumoniae subsp. pneumoniae MGH 78578 at 2.46 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-04 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.420, 105.507, 181.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.881 - 2.460 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.22800 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.698 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.881 | 29.881 | 2.520 |
High resolution limit [Å] | 2.460 | 11.000 | 2.460 |
Rmerge | 0.111 | 0.060 | 0.696 |
Total number of observations | 4964 | 37475 | |
Number of reflections | 68362 | ||
<I/σ(I)> | 4.443 | 9.4 | 1.1 |
Completeness [%] | 99.8 | 93 | 99.7 |
Redundancy | 7.5 | 6.2 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 31.0% polyethylene glycol 600, 0.1M CHES pH 9.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |