3F1X
Three dimensional structure of the serine acetyltransferase from Bacteroides vulgatus, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET BVR62.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97913, 0.96863, 0.97927 |
Spacegroup name | H 3 2 |
Unit cell lengths | 111.417, 111.417, 124.426 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.440 - 2.000 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.081 | 0.370 |
Number of reflections | 38687 | |
<I/σ(I)> | 24.7 | 9.4 |
Completeness [%] | 98.6 | 86.4 |
Redundancy | 11.1 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.2 | 277 | 0.88M Potassium nitrate, 0.1M Sodium citrate, pH 4.2, microbatch method, temperature 277K |