3F1V
E. coli Beta Sliding Clamp, 148-153 Ala Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-07-05 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.838, 64.471, 72.111 |
| Unit cell angles | 73.83, 82.72, 83.76 |
Refinement procedure
| Resolution | 28.860 - 1.770 |
| R-factor | 0.2186 |
| Rwork | 0.215 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 01k7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.079 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.850 | 1.860 |
| High resolution limit [Å] | 1.770 | 1.760 |
| Rmerge | 0.032 | 0.300 |
| Number of reflections | 59193 | |
| <I/σ(I)> | 10.5 | 6.4 |
| Completeness [%] | 91.1 | 91.1 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | reservior: 13% isopropanol, 100 mM CaCl2, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
