3F1P
Crystal structure of a high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.916, 82.565, 41.032 |
| Unit cell angles | 90.00, 106.02, 90.00 |
Refinement procedure
| Resolution | 26.926 - 1.170 |
| R-factor | 0.1412 |
| Rwork | 0.141 |
| R-free | 0.16650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b02 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.368 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.930 | 1.180 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.068 | 0.369 |
| Number of reflections | 76140 | |
| <I/σ(I)> | 31.2 | 2.1 |
| Completeness [%] | 95.8 | 64.3 |
| Redundancy | 4.7 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion combined with microseeding | 6.5 | 293 | 30% PEG3350, 0.1M BisTris, 0.05M Tris, 0.017M NaCl, 0.005M DTT, pH 6.5, vapor diffusion combined with microseeding, temperature 293K |
