3F1J
Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals RNA binding properties
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-20 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9500, 0.97920, 0.97950 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 143.570, 143.570, 143.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.540 - 2.650 |
| R-factor | 0.221 |
| Rwork | 0.217 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.092 | 0.671 |
| Number of reflections | 7570 | 1123 |
| <I/σ(I)> | 11.97 | 3.1 |
| Completeness [%] | 98.8 | 99.1 |
| Redundancy | 7.46 | 7.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% PEG 8000, 0.5M Li2SO4, 0.1M Tris/HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
