3F13
Crystal structure of putative nudix hydrolase family member from Chromobacterium violaceum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-26 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.950, 43.877, 55.967 |
| Unit cell angles | 73.47, 74.06, 62.66 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.26400 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.590 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.882 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.081 | 0.511 |
| Total number of observations | 19331 | |
| Number of reflections | 34975 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 97.2 | 95.8 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 19% PEG 3350, 600mM ammonium chloride, pH 7.0, Vapor diffusion, temperature 294K |
