3F10
Crystal structure of Clostridium Acetobutylicum 8-oxoguanine DNA glycosylase in complex with 8-oxoguanosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-07-02 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 138.500, 138.500, 138.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.790 - 2.300 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.790 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.081 | 0.533 |
Number of reflections | 20809 | 1373 |
<I/σ(I)> | 3.14 | |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 18.74 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 285 | 12% PEG-4000, 0.1M NaCitrate, 20%isopropanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |