3F0W
Human NUMB-like protein, phosphotyrosine interaction domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 55.150, 55.150, 99.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.460 - 2.700 |
| R-factor | 0.24223 |
| Rwork | 0.238 |
| R-free | 0.28115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wj1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.923 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.066 | 0.565 |
| Number of reflections | 5126 | |
| <I/σ(I)> | 19.28 | 4.03 |
| Completeness [%] | 99.1 | 98.7 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 277 | 24 % PEG 8000, 0.1M Lithium sulphate, 0.1M Sodium Acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
