3F0L
Crystal structure of oxidized D105N Synechocystis sp. PcyA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-31 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.845, 95.554, 42.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.620 - 1.300 |
R-factor | 0.17 |
Rwork | 0.169 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.721 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.620 | 1.330 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 72066 | |
Completeness [%] | 97.6 | 95.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | 1.45M Ammonium sulfate, 0.15M NaCl, 0.1M Aacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |