3F08
Crystal structure of the putative uncharacterized protein Q6HG14 from Bacilllus thuringiensis. Northeast Structural Genomics Consortium target BuR153.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-22 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 129.666, 129.666, 75.432 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.870 - 2.200 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cnw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.114 |
| Number of reflections | 35985 |
| <I/σ(I)> | 20.9 |
| Completeness [%] | 100.0 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M KH2PO4, 0.1M Na3-citrate 22% PEG1K, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
