3F08
Crystal structure of the putative uncharacterized protein Q6HG14 from Bacilllus thuringiensis. Northeast Structural Genomics Consortium target BuR153.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-22 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 129.666, 129.666, 75.432 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.870 - 2.200 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cnw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.114 |
Number of reflections | 35985 |
<I/σ(I)> | 20.9 |
Completeness [%] | 100.0 |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M KH2PO4, 0.1M Na3-citrate 22% PEG1K, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |