3F05
Crystal Structure of Synaptotagmin I C2A domain with Mn(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-03 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.549, 38.512, 44.058 |
| Unit cell angles | 90.00, 98.70, 90.00 |
Refinement procedure
| Resolution | 20.540 - 1.400 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22800 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.153 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 3.020 | 1.400 |
| Rmerge | 0.108 | 0.073 | 0.153 |
| Number of reflections | 26922 | ||
| <I/σ(I)> | 18.261 | ||
| Completeness [%] | 98.3 | 98.2 | 94.1 |
| Redundancy | 5.4 | 5.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2.0M Li2SO4, 100mM Tris, pH 7.5, vapor diffusion, hanging drop, temperature 298.0K |
