3EZN
Crystal structure of phosphoglyceromutase from burkholderia pseudomallei 1710b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-26 |
| Detector | SATURN 944 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.940, 49.080, 62.110 |
| Unit cell angles | 107.11, 91.19, 107.81 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x19 modified with ccp4 chainsaw |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.650 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.041 | 0.137 |
| Number of reflections | 26261 | |
| <I/σ(I)> | 25.7 | 9.9 |
| Completeness [%] | 93.7 | 83.2 |
| Redundancy | 3.74 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | EMERALD CRYO B-4: 100MM MES PH 6.0, 5% PEG 1000, 10% GLYCEROL, 30% PEG 600, PH 7.5, VAPOR DIFFUSION, TEMPERATURE 298K, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
