3EZI
Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain without Ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-04 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 28.241, 39.822, 83.947 |
Unit cell angles | 80.76, 89.57, 85.29 |
Refinement procedure
Resolution | 19.790 - 1.700 |
Rwork | 0.189 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.050 | 0.037 | 0.352 |
Number of reflections | 37137 | ||
<I/σ(I)> | 13.8 | ||
Completeness [%] | 92.3 | 98.3 | 78.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | isopropanol, PEG 3350, pH 7.5, vapor diffusion, temperature 277K |