3EZH

Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain in Complex with Nitrate

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-22
Wavelength(s)	0.9673, 0.9786, 0.9794, 0.9904
Spacegroup name	P 43 21 2
Unit cell lengths	55.760, 55.760, 155.257
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.940 - 1.700
Rwork	0.217
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.046	0.042	0.250
Number of reflections	27197
<I/σ(I)>	20.1
Completeness [%]	98.4	94.6	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	277	ammonium phosphate, pH 8.5, vapor diffusion, temperature 277K