3EZH
Crystal Structure of the E. coli Histidine Kinase NarX Sensor Domain in Complex with Nitrate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-22 |
| Wavelength(s) | 0.9673, 0.9786, 0.9794, 0.9904 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.760, 55.760, 155.257 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.940 - 1.700 |
| Rwork | 0.217 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.046 | 0.042 | 0.250 |
| Number of reflections | 27197 | ||
| <I/σ(I)> | 20.1 | ||
| Completeness [%] | 98.4 | 94.6 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 277 | ammonium phosphate, pH 8.5, vapor diffusion, temperature 277K |
