3EZ8
Crystal Structure of endoglucanase Cel9A from the thermoacidophilic Alicyclobacillus acidocaldarius
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.977 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 49.059, 84.967, 129.479 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.876 - 2.302 |
R-factor | 0.1969 |
Rwork | 0.197 |
R-free | 0.23340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1clc |
RMSD bond length | 0.008 |
RMSD bond angle | 1.061 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.876 | 2.340 |
High resolution limit [Å] | 2.302 | 2.300 |
Rmerge | 0.550 | |
Number of reflections | 23484 | |
<I/σ(I)> | 1.7 | |
Completeness [%] | 94.8 | 93.7 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 298 | 0.1 M HEPES and 55 % MPD, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K |