3EYZ
Cocrystal structure of Bacillus fragment DNA polymerase I with duplex DNA (open form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.663, 94.053, 106.925 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
Rwork | 0.211 |
R-free | 0.24500 |
Starting model (for MR) | 1l3s |
RMSD bond length | 0.006 |
RMSD bond angle | 1.158 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.084 | 0.041 | 0.408 |
Number of reflections | 49257 | ||
<I/σ(I)> | 10 | ||
Completeness [%] | 93.8 | 98.6 | 67 |
Redundancy | 3.8 | 4.2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | Ammonium Sulfate, MES, MPD, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Ammonium Sulfate | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 2 | Ammonium Sulfate | ||
5 | 1 | 2 | MES | ||
6 | 1 | 2 | MPD |