3EYY
Structural basis for the specialization of Nur, a nickel-specific Fur homologue, in metal sensing and DNA recognition
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.4847, 1.4857, 1.4510 |
| Spacegroup name | P 31 |
| Unit cell lengths | 79.164, 79.164, 49.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.790 - 2.400 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 18205 | |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 296.15 | 14% PEG 3350, 0.4M sodium malonate (pH 7), 0.1mM benzamidine hydrochloride, 0.1mM NiCl2, pH 7.0, Microbatch, temperature 296.15K |
