3EYV

Anti-Lewis Y Fab fragment with Lewis Y antigen in the presence of zinc ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-03-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	78.752, 101.467, 114.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.650 - 2.500
Rwork	0.210
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s3k
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	31623
<I/σ(I)>	23.8	4.5
Completeness [%]	95.8	94.7
Redundancy	5.5	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	25% (v/v) PEG MONOMETHYL ETHER 550, 0.1M MES, 0.01M ZINC SULFATE, pH 6.5, VAPOR DIFFUSION, temperature 291.0K