3EYU
PFA1 Fab fragment complexed with Ror2(518-525)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.632, 42.632, 58.729 |
| Unit cell angles | 96.09, 93.58, 91.71 |
Refinement procedure
| Resolution | 21.290 - 2.710 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.24800 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.246 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.290 | 2.778 |
| High resolution limit [Å] | 2.710 | 2.709 |
| Number of reflections | 9216 | |
| Completeness [%] | 84.8 | 45.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% w/v PEG3350, 0.2 M LiCl, vapor diffusion, sitting drop, temperature 298K |
