3EYT
Crystal structure of Thioredoxin-like superfamily protein SPOA0173
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97928 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.543, 76.800, 137.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.520 - 1.950 |
R-factor | 0.175 |
Rwork | 0.173 |
R-free | 0.21000 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.417 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.520 | 1.970 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.142 | 0.893 |
Number of reflections | 51070 | |
<I/σ(I)> | 20.256 | 1.7 |
Completeness [%] | 99.9 | 97 |
Redundancy | 7.1 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 0.2M Ammonium sulfate 0.1M Sodium acetate pH 4.6 12% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |