3EYI
The crystal structure of the second Z-DNA binding domain of human DAI (ZBP1) in complex with Z-DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9795, 0.9798, 0.9721 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.530, 58.249, 88.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.450 |
| R-factor | 0.15719 |
| Rwork | 0.153 |
| R-free | 0.19350 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.649 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.075 | 0.156 |
| Number of reflections | 26766 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 95.8 | 84.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M Tris-HCl pH 8.5, 31-33% PEG 4000, 200-260 mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris-HCl | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 1 | sodium acetate | ||
| 4 | 1 | 2 | Tris-HCl | ||
| 5 | 1 | 2 | PEG 4000 | ||
| 6 | 1 | 2 | sodium acetate |
