3EY7
Structure from the mobile metagenome of V. Cholerae. Integron cassette protein VCH_CASS1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.029, 67.868, 91.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.754 - 1.600 |
R-factor | 0.1738 |
Rwork | 0.171 |
R-free | 0.21640 |
Structure solution method | SAD |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.470 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.084 | 0.430 |
Number of reflections | 34682 | |
<I/σ(I)> | 15.8 | 3.7 |
Completeness [%] | 96.7 | 95.3 |
Redundancy | 7 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 294 | 9% PEG 8000, 0.2M Ca(OAC), 0.1M NA Cacodylate, pH 6.5, VAPOR DIFFUSION, temperature 294K |