3EY5
Putative acetyltransferase from GNAT family from Bacteroides thetaiotaomicron.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-08 |
Detector | SBC-3 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.798, 50.785, 73.765 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.300 - 2.150 |
R-factor | 0.201 |
Rwork | 0.198 |
R-free | 0.26700 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.519 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.300 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.113 | 0.549 |
Number of reflections | 9184 | |
<I/σ(I)> | 22.3 | 3.17 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 5.4 | 5.54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 277 | 0.2 M lithium sulfate, 0.1 M CAPS buffer, 1.2 M sodium/potassium phosphate, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |