3EXN
Crystal structure of acetyltransferase from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-13 |
| Detector | SBC-3 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.197, 52.408, 47.514 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.17825 |
| Rwork | 0.176 |
| R-free | 0.21899 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.589 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.194 |
| Number of reflections | 14476 | |
| <I/σ(I)> | 26.3 | 4.8 |
| Completeness [%] | 98.6 | 94.9 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 289 | 0.1 M lithium Chloride 25% Peg 6000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
