3EXA
Crystal structure of the full-length tRNA isopentenylpyrophosphate transferase (BH2366) from Bacillus halodurans, Northeast Structural Genomics Consortium target BhR41.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-10 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 |
Unit cell lengths | 51.805, 73.824, 95.561 |
Unit cell angles | 89.99, 93.73, 90.02 |
Refinement procedure
Resolution | 19.960 - 2.300 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.26300 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB (then SOLVE/RESOLVE) |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.900 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.040 | 0.088 |
Number of reflections | 89734 | |
<I/σ(I)> | 15.6 | 5.3 |
Completeness [%] | 70.7 | 55.1 |
Redundancy | 1.5 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Protein solution: 10 mM Tris-HCl pH (7.5), 100 mM NaCl, 5 mM DTT, and 0.025% (w/v) NaN3. Reservoir solution: 16% PEG 3350 and 200 mM Ammonium tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |