3EX9
Crystal structure of PhzA/B from Burkholderia cepacia R18194 crystallized in C2221
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 78.940, 79.680, 64.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.19476 |
Rwork | 0.192 |
R-free | 0.23968 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b4o |
RMSD bond length | 0.022 |
RMSD bond angle | 1.831 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.260 | 2.300 |
High resolution limit [Å] | 2.200 | 2.100 |
Rmerge | 0.429 | |
Number of reflections | 10338 | |
<I/σ(I)> | 20.1 | 5.5 |
Completeness [%] | 97.4 | 95.7 |
Redundancy | 9.2 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 284 | 0.1M Bis-TRIS, 0.2M NH4OAc, 20% (w/v) PEG 3350, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 284K |