3EWT
Crystal Structure of calmodulin complexed with a peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2006-09-22 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 40.062, 40.062, 174.431 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.900 - 2.400 |
R-factor | 0.21859 |
Rwork | 0.217 |
R-free | 0.25916 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l7z |
RMSD bond length | 0.011 |
RMSD bond angle | 1.384 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.042 | |
Number of reflections | 6946 | |
Completeness [%] | 99.6 | 97.3 |
Redundancy | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.5 | 293 | 25%(w/v) PEG8000, 0.2M Sodium Acetate, 0.1M Sodium cacodylate, pH 5.5, EVAPORATION, temperature 293K |