3EWM
Crystal structure of an uncharacterized sugar kinase PH1459 from pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.757, 79.373, 82.990 |
| Unit cell angles | 90.00, 94.18, 90.00 |
Refinement procedure
| Resolution | 57.260 - 1.900 |
| R-factor | 0.19217 |
| Rwork | 0.190 |
| R-free | 0.23906 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (& SHARP) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.260 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.145 | 0.307 |
| Number of reflections | 44270 | |
| <I/σ(I)> | 12 | 3.9 |
| Completeness [%] | 99.2 | 95.3 |
| Redundancy | 7.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 18% PEG 8000; 0.2M calcium acetate; 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
