3EWK
Structure of the redox sensor domain of Methylococcus capsulatus (Bath) MmoS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 146.710, 146.710, 43.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.800 - 2.340 |
R-factor | 0.24 |
Rwork | 0.238 |
R-free | 0.28300 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.969 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.800 | 3.030 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.073 | 0.230 |
Number of reflections | 17002 | |
<I/σ(I)> | 33.314 | |
Completeness [%] | 95.2 | 81.6 |
Redundancy | 12.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Ammonium phosphate and glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Ammonium phosphate and glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |