3EW7
Crystal structure of the Lmo0794 protein from Listeria monocytogenes. Northeast Structural Genomics Consortium target LmR162.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97894, 0.97926, 0.96785 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.768, 83.768, 92.215 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.760 - 2.730 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.106 | 0.648 |
| Number of reflections | 19111 | |
| <I/σ(I)> | 16.05 | 4.05 |
| Completeness [%] | 98.9 | 97.9 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | 18% PEG 4000, 0.1M KH(2)PO(4), 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
