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3EW0

The novel 2Fe-2S outer mitochondrial protein mitoNEET displays conformational flexibility in its N-terminal cytoplasmic tethering domain

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 12.3.1
Synchrotron siteALS
Beamline12.3.1
Temperature [K]100
Detector technologyCCD
Collection date2007-10-12
DetectorADSC QUANTUM 315
Wavelength(s)1.1158
Spacegroup nameP 21 21 21
Unit cell lengths50.737, 48.476, 59.245
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution38.550 - 1.400
R-factor0.20047
Rwork0.198
R-free0.24064
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2qh7
RMSD bond length0.011
RMSD bond angle1.577
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwareREFMAC (5.5.0063)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]38.5501.480
High resolution limit [Å]1.4001.400
Number of reflections26874
<I/σ(I)>194.5
Completeness [%]92.481.4
Redundancy6.34.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION8300Crystallization Droplets: 100 mM Tris HCl (pH 8.0), 100 mM NaCl, and 16-18% PEG 3000. Reservoir conditions: 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 30 32% PEG 3000 in the reservoir., VAPOR DIFFUSION

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