3EW0
The novel 2Fe-2S outer mitochondrial protein mitoNEET displays conformational flexibility in its N-terminal cytoplasmic tethering domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1158 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.737, 48.476, 59.245 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.550 - 1.400 |
R-factor | 0.20047 |
Rwork | 0.198 |
R-free | 0.24064 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qh7 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.577 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.550 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 26874 | |
<I/σ(I)> | 19 | 4.5 |
Completeness [%] | 92.4 | 81.4 |
Redundancy | 6.3 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 300 | Crystallization Droplets: 100 mM Tris HCl (pH 8.0), 100 mM NaCl, and 16-18% PEG 3000. Reservoir conditions: 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 30 32% PEG 3000 in the reservoir., VAPOR DIFFUSION |