3EVZ
Crystal structure of Methyltransferase from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-10-01 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 46.877, 46.877, 331.412 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.220 - 2.200 |
R-factor | 0.20195 |
Rwork | 0.199 |
R-free | 0.25900 |
Structure solution method | SAD |
RMSD bond length | 0.024 |
RMSD bond angle | 2.102 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.220 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.111 | 0.331 |
Number of reflections | 10605 | |
<I/σ(I)> | 29.1 | 8.6 |
Completeness [%] | 92.3 | 85 |
Redundancy | 28.4 | 27.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | D9. 0.1 M Tris pH 8.5, 25% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |