3EVY
Crystal structure of a fragment of a putative type I restriction enzyme R protein from Bacteroides fragilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.834, 56.470, 75.857 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.27400 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.695 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.314 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.114 | 0.590 |
| Total number of observations | 21701 | |
| Number of reflections | 17791 | |
| <I/σ(I)> | 9.5 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.4 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 20% PEG 3350, 200mM potassium sulfate, pH 7.0, Vapor diffusion, temperature 294K |
