3EVW
Crystal structure of the Mimivirus NDK R107G mutant complexed with dTDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2007-09-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.690, 151.038, 184.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.529 - 2.600 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b8q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.207 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.500 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 35004 | |
| <I/σ(I)> | 4.6 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 40 to 44% MPD, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
