3EVT
Crystal structure of phosphoglycerate dehydrogenase from Lactobacillus plantarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 75.848, 75.848, 107.834 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.232 |
Rwork | 0.230 |
R-free | 0.28800 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.634 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.932 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.096 | 0.370 |
Total number of observations | 52414 | |
Number of reflections | 16646 | |
<I/σ(I)> | 20.3 | 7.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 21.9 | 22.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 294 | 100mM Hepes pH 7.5, 8% ethylene glycol, 20% PEG 10K, Vapor diffusion, temperature 294K |