3EVS

Crystal structure of the GDF-5:BMP receptor IB complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.10485
Spacegroup name	P 42 21 2
Unit cell lengths	76.455, 76.455, 82.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.190 - 2.100
R-factor	0.217
Rwork	0.215
R-free	0.25600
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.339
Data reduction software	CrystalClear
Data scaling software	d*TREK (9.4SSI)
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.190	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.071	0.354
Total number of observations		14428
Number of reflections	14815
<I/σ(I)>		5.3
Completeness [%]	99.5	100
Redundancy	9.66	9.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.25	294	0.1M sodium acetate, 50% PPG 400, 30mM magnesium sulfate, pH 5.25, VAPOR DIFFUSION, temperature 294K
1	VAPOR DIFFUSION	5.25	294	0.1M sodium acetate, 50% PPG 400, 30mM magnesium sulfate, pH 5.25, VAPOR DIFFUSION, temperature 294K
1	VAPOR DIFFUSION	5.25	294	0.1M sodium acetate, 50% PPG 400, 30mM magnesium sulfate, pH 5.25, VAPOR DIFFUSION, temperature 294K