3EVM
Crystal structure of the Mimivirus NDK +Kpn-N62L-R107G triple mutant complexed with dCDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2007-09-24 |
| Detector | KODAK |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 |
| Unit cell lengths | 70.323, 70.323, 104.207 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.228 - 1.800 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.294 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 24190 | |
| <I/σ(I)> | 8.9 | 2.5 |
| Completeness [%] | 89.4 | 89.4 |
| Redundancy | 7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.8 to 1M Sodium Citrate, 0.1M Tris, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
