3EV3
Crystal Structure of Ribonuclease A in 70% t-Butanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.832, 32.712, 72.681 |
| Unit cell angles | 90.00, 90.64, 90.00 |
Refinement procedure
| Resolution | 31.120 - 1.680 |
| Rwork | 0.207 |
| R-free | 0.23300 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.220 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 3.620 | 1.680 |
| Rmerge | 0.131 | 0.068 | 0.264 |
| Number of reflections | 26786 | ||
| Completeness [%] | 97.2 | 96.1 | 93.4 |
| Redundancy | 2.7 | 2.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | PEG 4000, sodium citrate, pH 5.0, vapor diffusion, hanging drop, temperature 291K |
