3EV1
Crystal Structure of Ribonuclease A in 70% Hexanediol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.598, 32.708, 73.413 |
| Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.000 |
| Rwork | 0.235 |
| R-free | 0.29400 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.243 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
| Rmerge | 0.123 | 0.093 | 0.778 |
| Number of reflections | 15129 | ||
| Completeness [%] | 91.3 | 99.7 | 65.9 |
| Redundancy | 2.7 | 3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | PEG 4000, sodium citrate, pH 5.0, vapor diffusion, hanging drop, temperature 291K |
