3EUW
Crystal Structure of a Myo-inositol dehydrogenase from Corynebacterium glutamicum ATCC 13032
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.039, 127.586, 87.155 |
| Unit cell angles | 90.00, 92.96, 90.00 |
Refinement procedure
| Resolution | 38.210 - 2.300 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.25800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.114 | 0.431 |
| Number of reflections | 85247 | |
| <I/σ(I)> | 11 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 5% PEG 2000, 50% Ethylene glycol, 0.1 M Sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
