3EUP
The crystal structure of the transcriptional regulator, TetR family from Cytophaga hutchinsonii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.595, 53.400, 178.005 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.090 - 1.990 |
R-factor | 0.18463 |
Rwork | 0.182 |
R-free | 0.22937 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.631 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.090 | 2.046 |
High resolution limit [Å] | 1.990 | 2.000 |
Rmerge | 0.110 | 0.670 |
Number of reflections | 28745 | |
<I/σ(I)> | 31.62 | 2.76 |
Completeness [%] | 99.1 | 94.47 |
Redundancy | 9.2 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 25% PEG4000, 0.1M MES, 0.2M MgCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |